The aperture of the pipette can be as low as 0.1 μ m. But the tip is very fragile, so it is easy to be damaged during the sample preparation process. When preparing, it is necessary to be careful of the tip damage and ensure that the sealing around the pipette is intact after implantation.

? The tip of the pipette is used to produce defects of a specific diameter

? Use flow rate to authenticate leaks

? Geometric structure of tip defects